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葡聚糖凝膠G-50Sephadex G-50

CAS號：9048-71-9

英文名稱：Sephadex G-50
其他名稱：交聯(lián)葡聚糖凝膠G-50
CAS號：9048-71-9
干顆粒直徑：40～120um
分子量分級范圍：A.肽及球形蛋白質(zhì)：1500～30000；B. 葡聚糖（線性分子）：500～10000
床體積毫升/克干分子篩：9～11ml/g
得水值：5.0±0.3
溶脹zui少平衡時(shí)間（h）：室溫6h（20℃）；沸水浴2h（90℃）
PH：2～13
性狀：白色珠粒，屬親水性凝膠。性穩(wěn)定，不溶于水、弱酸和堿溶液，遇強(qiáng)酸能使糖苷鍵分解

葡聚糖凝膠G-50Sephadex G-50

Sephadex G-50 Medium, 100 g

Sephadex G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

• Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
• Suitable for purification of DNA from small molecules by gel filtration
• Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
• Classic gel filtration medium.

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

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